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General Information

Name Information
Code: T082
Test Name, Short: Cot , saliva
A.K.A.: COT, Saliva
Matrix: Saliva
Turnaround Time: Daily
Analytical Method: enzyme-linked immunosorbent assay (ELISA)
Reportable - Test Results Type: Qualitative

Specimen Information

Name Information
Min. Volume: The volume of oral fluid necessary for testing is 75 uL. However, there generally is a panel of four tests performed on oral fluid and there is a specific priority of testing when small volumes are encountered. Amylase and HIV have the first and second, then cocaine is third, and cotinine is fourth. A minimum of 190 µL of sample is required to perform all four tests.
Rejection Criteria: Grossly discolored samples or specimens that have gross particulate matter should have all tests cancelled with the comment NSA in Labware. Oral Fluid samples that do not contain adequate volume should have their tests cancelled in Labware as QNS.
Collection Instructions: Saliva from an OraSure® Oral Specimen Collection Device is the recommended specimen. The consent, collection device, and a coordinating clean polypropylene 16 x 75 tube is barcoded by ILS setups. ILS Setups then prepares the sample for testing as follows. The collection device is held with the tip in the upright position, and then the tip is broken off. This end is then mounted onto the empty polypropylene tube. The original swab collection device and empty tube are then spun in the centrifuge at 2000 rpms for 5 minutes. The actual saliva testing specimen transfers to the polypropylene tube during the centrifugation process. The tubes are pulled apart from each other. The actual testing saliva specimens are racked into Hamilton primary racks for initial testing while the corresponding swab is racked in cardboard boxes mirroring the Hamilton rack.
Collection Patient Prep: NA
Container: Collect saliva samples according to instructions provided with the collection kit.
Shipping Temperature: Ambient